ONLINE ANALYSIS TOOLS |
Each of the items in blue text is hyperlinked to a separate page containing useful sites on the Web.
Analysis of nucleotide and protein sequence data was initially restricted to
those with access to complicated mainframe
or expensive desktop computer
programs (for example PC/GENE, Lasergene, MacVector,
Accelrys etc.). The availability
of
online tools permits even the novice
molecular biologist the opportunity to derive
a considerable amount of useful nformation from nucleotide or protein sequence
data. For those with no experience I have provided three sequences:
(a) a DNA sequence, (b) a
protein sequence, and (c) four protein sequences presented in
FASTA format.
Prior to trying
out a Web Site select the sequence and copy to clipboard.
Each of the items in blue text is hyperlinked to a site on the
Web. Each of these Web Sites has a box into which you can
"Paste" your sequence. Click on the button labeled
"Search,"
"Run" or "Submit." If in doubt use the default setting that
the sites provide, but for the more adventuresome
some of the sites offer the chance of modifying the search strategy.

ONLINE RESOURCES (tutorials and glossaries)


ALIGNMENTS
CONVERT SEQUENCE (upper to low case, complement, reverse, RNA to DNA)
COMPOSITION
DNA MOTIFS
HOMOLOGY SEARCHES
MELTING TEMPERATURE (Tm)
PCR PRIMER DESIGN
PHYLOGENY
PROMOTERS
REPEATS & SECONDARY STRUCTURE
RESTRICTION ENDONUCLEASE SITES
TRANSLATION (GENERAL)
TRANSLATION (EUKARYOTIC GENES) (identification of introns and exons)
TRANSCRIPTION FACTORS


ALIGNMENTS
COMPOSITION & CHEMISTRY
HOMOLOGY SEARCHES
MOTIFS
PHYLOGENY
QUATERNARY STRUCTURE PREDICTIONS
SECONDARY STRUCTURE PREDICTIONS
TERTIARY STRUCTURE PREDICTIONS



BUFFERS
CENTRIFUGATION CALCULATIONS
GENERAL MOLECULAR BIOLOGY CALCULATIONS
META SITES FOR DNA & PROTEIN ANALYSIS
SEQUENCE CLEANUP & CONVERSION
ONLINE GRAPHICS
WINDOWS- & JAVA or PERL-BASED PROGRAMS - free molecular biology programs
RECOVERING SEQUENCES FROM GENBANK
The Bio-Web: Resources for Molecular and Cell Biologists is a non-commercial, educational site with the only purpose of facilitating access to biology-related information over the internet.
CREATED BY: Dr. Andrew Kropinski, Molecular & Cellular Biology, University of Guelph, Guelph , Ontario, N1G 2W1 CANADA; and, Public Health Agency of Canada's Laboratory for Foodborne Zoonoses, Guelph, Ontario, N1G 3W4 CANADA.
Problems and suggestions: Contact Dr. Andrew Kropinski
LAST UPDATE: February 4, 2011